Investigation of reducing omniscan toxicity using intracellular and targeted n-acetylcysteine –lysine complex

Hadi Hejazinia,1 Mohammad esfandiar,2 Mehdi shafiee ardestani,3,*

1. Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
2. Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Abstract


Introduction

The reduction in toxicity at the dosage of 100 μm has been shown as the greatest reduction the amount of renal toxicity was reported to be 40 %.

Methods

Contrast gadodiamide (omniscan)(a medication from ge health care corkireland) n-acetyl cysteine (germany) l-lysine (germany) nn-dicyclohexylcarbodiimide 98% (dcc)( merck germany)dimethylformamide (dmf) mother (major) solvent (dimethyl sulfoxide (dmso) (merckgermany) chloroform solvent(merck germany) 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide(edc)(sigma usa) sulfo-nhs (–n-hydroxysulfosuccinimide)(sigma usa)calcium chloride(merck germany) trypan blue

Results

We designed this method in order to reduce toxic effects of contrast agent (omniscan) on kidney cells. nsf has been one of the main challenges in the field of contrast agents. furthermore our main goal is to reduce the toxic effects of contrast agents. according to the past studies nac and lysine were used more to the point after the design of the complex and determine the structure of the complex cell testing in order to assessment of cell toxicity reduction on the hek 293 cells done. nevertheless cytotoxicity decreased significantly

Conclusion

One of the most important and challenging problems in using contrast materials (agents) in mri and ct scan is nsf. in order to reduce the toxicity and increase permeability of omniscan into the target cells conjugated compound nac-lysine has been used for which nac molecules are connected with each other and from the other side to l-lysine amino acid through an amide bond

Keywords

Cancer n-acetylcysteinelysine nmr, reduced toxicity and increased permeability of omniscan