Investigation on the effects of raloxifene on spatial memory of rat

Shirin Hatami,1,* Mahdieh anoush,2

1. zanjan university of medical science
2. zanjan university of medical science

Abstract


Introduction

Regarding to the importance of alzheimer’s disease incidence in high aged adults it seems necessary to work on the prophylactic methods. considering the role of estrogen and it’s modulators in alzheimer progress and the neuroprotective effect for raloxifene; our purpose was to investigate the probable medical effects of raloxifene on alzheimer’s model in male rats.

Methods

Memory impairment induced by injection of scopolamine and the prophylactic, co-treatment and post treatment effects of raloxifene using both behavioral and quantitative methods were evaluated. morris water maze was used for spatial memory. soon after the test was fulfilled, the rats were decapitated, the brains were separated and half of them were drowned for histopathologic studies and half were put in order to acetylcholine content determination.

Results

The results obtained from behavioral study demonstrated that raloxifene was effective both using as pretreatment and co-treatment. also ach concentration was augmented in the groups received raloxifene. the histopathologic studies also demonstrated that raloxifene was able to reduce cell death and inflammation. none of the above mentioned effects was reported with pos-treatment therapy with raloxifene.

Conclusion

According to the results it can be deducted that raloxifene treatment both before and at the same time with scopolamine, caused marked improvements not only at learning patterns but also on ach concentration, cell death and inflammation. therefore, this study strengthens the hypothesis that chronic raloxifene treatment is capable of prophylaxis of diseases related with memory impairment such as alzheimer.

Keywords

Raloxifene, scopolamine, spatial memory, acetylcholine, morris water maze