Elimination of multidrug-resistant mycobacterium tuberculosis with silver, zinc oxide and mixture nanoparticles

Alireza Jafari,1,* Ali alavi foumani,2 Rajendra goswami,3 Jasper marc gamolo bondoc,4 Rui ma ,5 Nader mosavari,6

1. Inflammatory Lung Disease Research Center, Department of Internal Medicine, Razi Hospital, School of Medicine, Guilan Un
2. Inflammatory Lung Disease Research Center, Department of Internal Medicine, Razi Hospital, School of Medicine, Guilan Un
3. Institute for Tuberculosis Research, College of Pharmacy, University of Illinois at Chicago, Chicago, Illinois, USA
4. Institute for Tuberculosis Research, College of Pharmacy, University of Illinois at Chicago, Chicago, Illinois, USA
5. Institute for Tuberculosis Research, College of Pharmacy, University of Illinois at Chicago, Chicago, Illinois, USA
6. Reference Laboratory for Bovine Tuberculosis, Razi Vaccine and Serum Research Institute, Agricultural Research Education

Abstract


Introduction

Discovery in terms of novel compounds to eliminate multidrug-resistant mycobacterium tuberculosis (mdr-tb) had become an emergence. the aim was evaluated of anti-tubercular and toxicity impact of mixture silver (ag) and zinc oxide (zno) nanoparticles (nps).

Methods

Anti-tubercular impact of nps was done by micro-plate alamar blue, and proportion methods against mdr-tb. the toxicity of nps also was evaluated by mtt assay against hepg2 and vero cell lines.

Results

Ag nps could not kill mdr-tb. the half maximal inhibitory concentration (ic50) against mdr-tb were 0.779 ppm for zno nps and 0.883 ppm for agzno nps.

Conclusion

The agzno nps was not toxic against vero cell lines. we found that both zno and agzno nps have higher bactericide behavior against mdr-tb.

Keywords

Multidrug-resistant tuberculosis; hep g2 cell; vero cell; metal nanocrystals